This page was produced as an assignment for Genetics 677
Intragenic deletion of LIMK1 in mice
LIMK1 can be knocked out in mice to create a partial Williams Syndrome phenotype.
To do this, one must first construct a vector (Fig 2) and then inject the embryonic stem cells containing the vector into the blastocyst, as shown on the right (Fig 1). Then, this blastocyst must be injected into a pseudo-pregnant mother. The offspring will have tissue contributions from both the mother and the blastocyst containing the knockout. Mice must then be selected (usually by color) and bred to create a pure knockout. Figure 2. Creating a vector (2).
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Figure 1. Creating a transgenic mouse (2).
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The phenotype seen in knockout LIMK1 mice is shown below:
Figure 3. Knockout LIMK1 mouse phenotype (1).
The abnormal learning/memory can be linked with lack of visuospatial cognition, which I choose to examine in my experiment proposal.
References
1.http://www.informatics.jax.org/javawi2/servlet/WIFetch?page=alleleDetail&key=25394#phenotypeSummary
2.http://www.scq.ubc.ca/studying-gene-function-creating-knockout-mice/
Last updated by Natalie DeCheck on May 22, 2012
1.http://www.informatics.jax.org/javawi2/servlet/WIFetch?page=alleleDetail&key=25394#phenotypeSummary
2.http://www.scq.ubc.ca/studying-gene-function-creating-knockout-mice/
Last updated by Natalie DeCheck on May 22, 2012